| SPACER | COLOR | SEQUENCE |
| spacer1 | BurlyWood | GACAGAACGGCCTCAGTAGTCTCGTCAGGCTC |
| spacer2 | Orange | CTGTTTTCGCAAATCTATGGACTATTGCTATT |
| spacer3 | Orchid | TGGCTCTGCAACAGCAGCACCCATGACCACGT |
| spacer4 | PaleVioletRed | GAAATGCTGGTGAGCGTTAATGCCGCAAACAC |
| spacer5 | CadetBlue | ATTACGCCTTTTTGCGATTGCCCGGTTTTTGC |